Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
Identifieur interne : 005F24 ( Main/Exploration ); précédent : 005F23; suivant : 005F25Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
Auteurs : Qin-Fen Zhang [République populaire de Chine] ; Jin-Ming Cui ; Xiao-Jun Huang ; Wei Lin ; Dong-Yan Tan ; Jie-Wei Xu ; Yi-Feng Yang ; Jing-Qiang Zhang ; Xin Zhang ; Hui Li ; Huan-Ying Zheng ; Qiu-Xia Chen ; Xin-Ge Yan ; Kui Zheng ; Zhuo-Yue Wan ; Jie-Cheng HuangSource :
- Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [ 0582-9879 ] ; 2003.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero (virologie), Enveloppe nucléaire (virologie), Humains, Larynx (virologie), Microscopie électronique, Noyau de la cellule (virologie), Partie nasale du pharynx (virologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Virion (ultrastructure), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification), Virus du SRAS (ultrastructure).
- MESH :
- immunologie : Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Cellules Vero, Enveloppe nucléaire, Larynx, Noyau de la cellule, Partie nasale du pharynx, Syndrome respiratoire aigu sévère.
- ultrastructure : Animaux, Humains, Microscopie électronique, Virion, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Cell Nucleus (virology), Chlorocebus aethiops, Humans, Larynx (virology), Microscopy, Electron, Nasopharynx (virology), Nuclear Envelope (virology), SARS Virus (immunology), SARS Virus (isolation & purification), SARS Virus (ultrastructure), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Vero Cells (virology), Virion (ultrastructure).
- MESH :
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- ultrastructure : SARS Virus, Virion.
- virology : Cell Nucleus, Larynx, Nasopharynx, Nuclear Envelope, Severe Acute Respiratory Syndrome, Vero Cells.
- Animals, Chlorocebus aethiops, Humans, Microscopy, Electron.
Abstract
After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.
PubMed: 12796822
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003343
- to stream PubMed, to step Curation: 003343
- to stream PubMed, to step Checkpoint: 003174
- to stream Ncbi, to step Merge: 000153
- to stream Ncbi, to step Curation: 000153
- to stream Ncbi, to step Checkpoint: 000153
- to stream Main, to step Merge: 006411
- to stream Main, to step Curation: 005F24
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.</title><author><name sortKey="Zhang, Qin Fen" sort="Zhang, Qin Fen" uniqKey="Zhang Q" first="Qin-Fen" last="Zhang">Qin-Fen Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Cui, Jin Ming" sort="Cui, Jin Ming" uniqKey="Cui J" first="Jin-Ming" last="Cui">Jin-Ming Cui</name></author><author><name sortKey="Huang, Xiao Jun" sort="Huang, Xiao Jun" uniqKey="Huang X" first="Xiao-Jun" last="Huang">Xiao-Jun Huang</name></author><author><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name></author><author><name sortKey="Tan, Dong Yan" sort="Tan, Dong Yan" uniqKey="Tan D" first="Dong-Yan" last="Tan">Dong-Yan Tan</name></author><author><name sortKey="Xu, Jie Wei" sort="Xu, Jie Wei" uniqKey="Xu J" first="Jie-Wei" last="Xu">Jie-Wei Xu</name></author><author><name sortKey="Yang, Yi Feng" sort="Yang, Yi Feng" uniqKey="Yang Y" first="Yi-Feng" last="Yang">Yi-Feng Yang</name></author><author><name sortKey="Zhang, Jing Qiang" sort="Zhang, Jing Qiang" uniqKey="Zhang J" first="Jing-Qiang" last="Zhang">Jing-Qiang Zhang</name></author><author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name></author><author><name sortKey="Li, Hui" sort="Li, Hui" uniqKey="Li H" first="Hui" last="Li">Hui Li</name></author><author><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name></author><author><name sortKey="Chen, Qiu Xia" sort="Chen, Qiu Xia" uniqKey="Chen Q" first="Qiu-Xia" last="Chen">Qiu-Xia Chen</name></author><author><name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name></author><author><name sortKey="Zheng, Kui" sort="Zheng, Kui" uniqKey="Zheng K" first="Kui" last="Zheng">Kui Zheng</name></author><author><name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name></author><author><name sortKey="Huang, Jie Cheng" sort="Huang, Jie Cheng" uniqKey="Huang J" first="Jie-Cheng" last="Huang">Jie-Cheng Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12796822</idno><idno type="pmid">12796822</idno><idno type="wicri:Area/PubMed/Corpus">003343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003343</idno><idno type="wicri:Area/PubMed/Curation">003343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003343</idno><idno type="wicri:Area/PubMed/Checkpoint">003174</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003174</idno><idno type="wicri:Area/Ncbi/Merge">000153</idno><idno type="wicri:Area/Ncbi/Curation">000153</idno><idno type="wicri:Area/Ncbi/Checkpoint">000153</idno><idno type="wicri:doubleKey">0582-9879:2003:Zhang Q:morphology:and:morphogenesis</idno><idno type="wicri:Area/Main/Merge">006411</idno><idno type="wicri:Area/Main/Curation">005F24</idno><idno type="wicri:Area/Main/Exploration">005F24</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.</title><author><name sortKey="Zhang, Qin Fen" sort="Zhang, Qin Fen" uniqKey="Zhang Q" first="Qin-Fen" last="Zhang">Qin-Fen Zhang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Lab for Biocontrol, Zhongshan University, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Cui, Jin Ming" sort="Cui, Jin Ming" uniqKey="Cui J" first="Jin-Ming" last="Cui">Jin-Ming Cui</name></author><author><name sortKey="Huang, Xiao Jun" sort="Huang, Xiao Jun" uniqKey="Huang X" first="Xiao-Jun" last="Huang">Xiao-Jun Huang</name></author><author><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name></author><author><name sortKey="Tan, Dong Yan" sort="Tan, Dong Yan" uniqKey="Tan D" first="Dong-Yan" last="Tan">Dong-Yan Tan</name></author><author><name sortKey="Xu, Jie Wei" sort="Xu, Jie Wei" uniqKey="Xu J" first="Jie-Wei" last="Xu">Jie-Wei Xu</name></author><author><name sortKey="Yang, Yi Feng" sort="Yang, Yi Feng" uniqKey="Yang Y" first="Yi-Feng" last="Yang">Yi-Feng Yang</name></author><author><name sortKey="Zhang, Jing Qiang" sort="Zhang, Jing Qiang" uniqKey="Zhang J" first="Jing-Qiang" last="Zhang">Jing-Qiang Zhang</name></author><author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name></author><author><name sortKey="Li, Hui" sort="Li, Hui" uniqKey="Li H" first="Hui" last="Li">Hui Li</name></author><author><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name></author><author><name sortKey="Chen, Qiu Xia" sort="Chen, Qiu Xia" uniqKey="Chen Q" first="Qiu-Xia" last="Chen">Qiu-Xia Chen</name></author><author><name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name></author><author><name sortKey="Zheng, Kui" sort="Zheng, Kui" uniqKey="Zheng K" first="Kui" last="Zheng">Kui Zheng</name></author><author><name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name></author><author><name sortKey="Huang, Jie Cheng" sort="Huang, Jie Cheng" uniqKey="Huang J" first="Jie-Cheng" last="Huang">Jie-Cheng Huang</name></author></analytic><series><title level="j">Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica</title><idno type="ISSN">0582-9879</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Nucleus (virology)</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Larynx (virology)</term><term>Microscopy, Electron</term><term>Nasopharynx (virology)</term><term>Nuclear Envelope (virology)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (ultrastructure)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vero Cells (virology)</term><term>Virion (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero (virologie)</term><term>Enveloppe nucléaire (virologie)</term><term>Humains</term><term>Larynx (virologie)</term><term>Microscopie électronique</term><term>Noyau de la cellule (virologie)</term><term>Partie nasale du pharynx (virologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virion (ultrastructure)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules Vero</term><term>Enveloppe nucléaire</term><term>Larynx</term><term>Noyau de la cellule</term><term>Partie nasale du pharynx</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Nucleus</term><term>Larynx</term><term>Nasopharynx</term><term>Nuclear Envelope</term><term>Severe Acute Respiratory Syndrome</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Microscopy, Electron</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Microscopie électronique</term><term>Virion</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">After infecting the Vero E6 cells by nasal/throat swabs collected from SARS patients, we studied the SARS-associated virus by electron microscopy and molecular biological technique. The results show that the diameter of newly isolated virus is about 50 nm without envelope or 100 nm with envelope. The virus was proved to be a new coronavirus by RT-PCR and it responded positively to convalescent-phase serum specimen from SARS patients, which is the evidence that this new virus is etiologically linked to the outbreak of SARS. The morphogenesis and distribution of the virus are also discussed in this article.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Chen, Qiu Xia" sort="Chen, Qiu Xia" uniqKey="Chen Q" first="Qiu-Xia" last="Chen">Qiu-Xia Chen</name><name sortKey="Cui, Jin Ming" sort="Cui, Jin Ming" uniqKey="Cui J" first="Jin-Ming" last="Cui">Jin-Ming Cui</name><name sortKey="Huang, Jie Cheng" sort="Huang, Jie Cheng" uniqKey="Huang J" first="Jie-Cheng" last="Huang">Jie-Cheng Huang</name><name sortKey="Huang, Xiao Jun" sort="Huang, Xiao Jun" uniqKey="Huang X" first="Xiao-Jun" last="Huang">Xiao-Jun Huang</name><name sortKey="Li, Hui" sort="Li, Hui" uniqKey="Li H" first="Hui" last="Li">Hui Li</name><name sortKey="Lin, Wei" sort="Lin, Wei" uniqKey="Lin W" first="Wei" last="Lin">Wei Lin</name><name sortKey="Tan, Dong Yan" sort="Tan, Dong Yan" uniqKey="Tan D" first="Dong-Yan" last="Tan">Dong-Yan Tan</name><name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name><name sortKey="Xu, Jie Wei" sort="Xu, Jie Wei" uniqKey="Xu J" first="Jie-Wei" last="Xu">Jie-Wei Xu</name><name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name><name sortKey="Yang, Yi Feng" sort="Yang, Yi Feng" uniqKey="Yang Y" first="Yi-Feng" last="Yang">Yi-Feng Yang</name><name sortKey="Zhang, Jing Qiang" sort="Zhang, Jing Qiang" uniqKey="Zhang J" first="Jing-Qiang" last="Zhang">Jing-Qiang Zhang</name><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name><name sortKey="Zheng, Kui" sort="Zheng, Kui" uniqKey="Zheng K" first="Kui" last="Zheng">Kui Zheng</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zhang, Qin Fen" sort="Zhang, Qin Fen" uniqKey="Zhang Q" first="Qin-Fen" last="Zhang">Qin-Fen Zhang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005F24 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005F24 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12796822
|texte= Morphology and morphogenesis of severe acute respiratory syndrome (SARS)-associated virus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12796822" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |